Genome sequencing is the process of determining the complete DNA sequence of an organism's genome . This technology has revolutionized the field of genetics and has become an indispensable tool in many areas of biological research and medicine. In this article, we will discuss the basic principles of genome sequencing, its applications, and its impact on various fields of study. The Human Genome Project (HGP) was the first large-scale genome sequencing project, which aimed to sequence the entire human genome. It was completed in 2003 , and since then, the cost of sequencing has significantly decreased, making genome sequencing more accessible to researchers and clinicians. Today, genome sequencing can be performed on a range of organisms, from bacteria to plants to animals, and even humans. Genome sequencing typically involves three main steps: Sample preparation Sequencing Data analysis Sample preparation involves extracting DNA from the organism or tissue of intere...